Course Name | Cell Culture Techniques |
Code | Semester | Theory (hour/week) | Application/Lab (hour/week) | Local Credits | ECTS |
---|---|---|---|---|---|
GBE 403 | Fall/Spring | 2 | 2 | 3 | 6 |
Prerequisites | None | |||||
Course Language | English | |||||
Course Type | Elective | |||||
Course Level | - | |||||
Mode of Delivery | - | |||||
Teaching Methods and Techniques of the Course | ||||||
Course Coordinator | ||||||
Course Lecturer(s) | - | |||||
Assistant(s) | - |
Course Objectives | The aim of this course is to use cell culture techniques, to be able to work under sterile conditions in cell culture |
Learning Outcomes | The students who succeeded in this course;
|
Course Description | Cell culture, Sterile conditions, Primary cell culture, Cell lines, Culture of stem cells and tumor cells, Three-dimensional cell culture |
Related Sustainable Development Goals | |
| Core Courses | |
Major Area Courses | ||
Supportive Courses | ||
Media and Managment Skills Courses | ||
Transferable Skill Courses |
Week | Subjects | Required Materials |
1 | Biology of Cultured Cells | Culture of Animal Cells-Chapter 2 |
2 | Laboratory Design, Layout, Equipment and Materials | Culture of Animal Cells-Chapter 3 and 4 |
3 | Aseptic Technique, Safety, Bioethics and Validation | Culture of Animal Cells-Chapter 5 and 6 |
4 | Culture Vessels and Substrates and Defined and Serum-Free Media | Culture of Animal Cells-Chapter 7, 8 and 9 |
5 | Preparation and Sterilization | Culture of Animal Cells-Chapter 10 |
6 | Primary Culture | Culture of Animal Cells-Chapter 11 |
7 | 1.Midterm | |
8 | Subculture and Cell Lines | Culture of Animal Cells-Chapter 12 |
9 | Cloning and Selection | Culture of Animal Cells-Chapter 13 |
10 | Cell Separation and Characterization | Culture of Animal Cells-Chapter 14 and 15 |
11 | Differentiation, Transformation and Immortalization | Culture of Animal Cells-Chapter 16 and 17 |
12 | 2.Midterm | |
13 | Contamination and Cryopreservation | Culture of Animal Cells-Chapter 18 and 19 |
14 | Stem Cells, Germ Cells and Amniocytes | Culture of Animal Cells-Chapter 23 |
15 | Culture of Tumor Cells and Three-Dimensional Culture | Culture of Animal Cells-Chapter 24 and 25 |
16 | Final exam |
Course Notes/Textbooks | Culture of Animal Cells, A Manual of Basic Technique and Specialized Applications 6th ed. By R. Ian Freshney. John Wiley&Sons (2010). |
Suggested Readings/Materials |
Semester Activities | Number | Weigthing |
Participation | ||
Laboratory / Application | ||
Field Work | ||
Quizzes / Studio Critiques | 2 | 10 |
Portfolio | ||
Homework / Assignments | 2 | 10 |
Presentation / Jury | 1 | 15 |
Project | ||
Seminar / Workshop | ||
Oral Exam | ||
Midterm | 2 | 40 |
Final Exam | 1 | 25 |
Total |
Weighting of Semester Activities on the Final Grade | 75 | |
Weighting of End-of-Semester Activities on the Final Grade | 25 | |
Total |
Semester Activities | Number | Duration (Hours) | Workload |
---|---|---|---|
Course Hours (Including exam week: 16 x total hours) | 16 | 2 | 32 |
Laboratory / Application Hours (Including exam week: 16 x total hours) | 16 | 2 | |
Study Hours Out of Class | 14 | 1 | 14 |
Field Work | |||
Quizzes / Studio Critiques | 2 | 5 | |
Portfolio | |||
Homework / Assignments | 2 | 5 | |
Presentation / Jury | 1 | 10 | |
Project | |||
Seminar / Workshop | |||
Oral Exam | |||
Midterms | 2 | 25 | |
Final Exams | 1 | 22 | |
Total | 180 |
# | Program Competencies/Outcomes | * Contribution Level | ||||
1 | 2 | 3 | 4 | 5 | ||
1 | To have adequate knowledge in Mathematics, Science and Biomedical Engineering; to be able to use theoretical and applied information in these areas on complex engineering problems. | |||||
2 | To be able to identify, define, formulate, and solve complex Biomedical Engineering problems; to be able to select and apply proper analysis and modeling methods for this purpose. | |||||
3 | To be able to design a complex system, process, device or product under realistic constraints and conditions, in such a way as to meet the requirements; to be able to apply modern design methods for this purpose. | |||||
4 | To be able to devise, select, and use modern techniques and tools needed for analysis and solution of complex problems in Biomedical Engineering applications. | |||||
5 | To be able to design and conduct experiments, gather data, analyze and interpret results for investigating complex engineering problems or Biomedical Engineering research topics. | |||||
6 | To be able to work efficiently in Biomedical Engineering disciplinary and multi-disciplinary teams; to be able to work individually. | |||||
7 | To be able to communicate effectively in Turkish, both orally and in writing; to be able to author and comprehend written reports, to be able to prepare design and implementation reports, to present effectively, to be able to give and receive clear and comprehensible instructions. | |||||
8 | To have knowledge about global and social impact of Biomedical Engineering practices on health, environment, and safety; to have knowledge about contemporary issues as they pertain to engineering; to be aware of the legal ramifications of engineering solutions. | |||||
9 | To be aware of ethical behavior, professional and ethical responsibility; to have knowledge about standards utilized in engineering applications. | |||||
10 | To have knowledge about industrial practices such as project management, risk management, and change management; to have awareness of entrepreneurship and innovation; to have knowledge about sustainable development. | |||||
11 | To be able to collect data in the area of Biomedical Engineering, and to be able to communicate with colleagues in a foreign language. | |||||
12 | To be able to speak a second foreign language at a medium level of fluency efficiently. | |||||
13 | To recognize the need for lifelong learning; to be able to access information, to be able to stay current with developments in science and technology; to be able to relate the knowledge accumulated throughout the human history to Biomedical Engineering. |
*1 Lowest, 2 Low, 3 Average, 4 High, 5 Highest